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pgl3 nfkb reporter  (Merck KGaA)

 
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    Structured Review

    Merck KGaA pgl3 nfkb reporter
    Pgl3 Nfkb Reporter, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+nfkb+reporter/pgl3+cmv++vector+cmv+/pm26358505-170-10-21
    Average 90 stars, based on 1 article reviews
    pgl3 nfkb reporter - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: CCL2 is transcriptionally controlled by the lysosomal protease cathepsin S in a CD74-dependent manner.
    Article Snippet: The catalytic cysteine residue of pcDNA3.1 CatS was mutated to a serine reside using site directed mutagenesis (Agilent Technologies, USA) to give rise to the CatS C/S construct. .. Cells were seeded in 6-well plates and transfected with a pGL3 NFkB reporter, Renilla, CCL2 promoter or control plasmid using Genejuice (Merck Millipore, UK) according to manufactures instructions. .. Cells were incubated for 24 hrs following transfection before lysis with passive lysis buffer and luciferase activity was determined using Dual-Glo® luciferase assay system (Promega, UK) as per manufactures instructions.

    Control:

    Article Title: CCL2 is transcriptionally controlled by the lysosomal protease cathepsin S in a CD74-dependent manner.
    Article Snippet: The catalytic cysteine residue of pcDNA3.1 CatS was mutated to a serine reside using site directed mutagenesis (Agilent Technologies, USA) to give rise to the CatS C/S construct. .. Cells were seeded in 6-well plates and transfected with a pGL3 NFkB reporter, Renilla, CCL2 promoter or control plasmid using Genejuice (Merck Millipore, UK) according to manufactures instructions. .. Cells were incubated for 24 hrs following transfection before lysis with passive lysis buffer and luciferase activity was determined using Dual-Glo® luciferase assay system (Promega, UK) as per manufactures instructions.

    Plasmid Preparation:

    Article Title: CCL2 is transcriptionally controlled by the lysosomal protease cathepsin S in a CD74-dependent manner.
    Article Snippet: The catalytic cysteine residue of pcDNA3.1 CatS was mutated to a serine reside using site directed mutagenesis (Agilent Technologies, USA) to give rise to the CatS C/S construct. .. Cells were seeded in 6-well plates and transfected with a pGL3 NFkB reporter, Renilla, CCL2 promoter or control plasmid using Genejuice (Merck Millipore, UK) according to manufactures instructions. .. Cells were incubated for 24 hrs following transfection before lysis with passive lysis buffer and luciferase activity was determined using Dual-Glo® luciferase assay system (Promega, UK) as per manufactures instructions.



    Similar Products

    pgl3 nfkb reporter  (Merck KGaA)
    90
    Merck KGaA pgl3 nfkb reporter
    Pgl3 Nfkb Reporter, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+nfkb+reporter/pgl3+cmv++vector+cmv+/pm26358505-170-10-21
    Average 90 stars, based on 1 article reviews
    pgl3 nfkb reporter - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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